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barcode oligonucleotides  (New England Biolabs)


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    Structured Review

    New England Biolabs barcode oligonucleotides
    Barcode Oligonucleotides, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/barcode+oligonucleotides/Nuclease-free+Water/pm41776501-44-26-36
    Average 99 stars, based on 4479 article reviews
    barcode oligonucleotides - by Bioz Stars, 2026-10
    99/100 stars

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    Plasmid Preparation:

    Article Title: Clonal dynamics shaped by diverse drug-tolerant persister states in melanoma resistance.
    Article Snippet: .. We digested the vector for 5 h with KpnI (NEB), gel-purified the linearized vector, and performed Gibson Assembly using 30 fmol of vector, 160 fmol of barcode oligonucleotides (100 nM in nuclease-free water), 15 μl of NEBuilder HiFi DNA Assembly Master Mix (NEB E2621), and nuclease-free water to a final volume of 30 μl, incubating at 50 °C for 1 h. The assembled plasmid was column-purified (Monarch PCR & DNA Cleanup Kit, NEB T1030) and then 25 ng of the column-purified plasmid was electroporated into 25 μl of Endura Electrocompetent Cells (Lucigen 60242-1) using a Gene Pulser II (Bio-Rad) under the following conditions: 10 μF capacitance, 600 Ohms resistance, 1.8 .. We digested the vector for 5 h with KpnI (NEB), gel-purified the linearized vector, and performed Gibson Assembly using 30 fmol of vector, 160 fmol of barcode oligonucleotides (100 nM in nuclease-free water), 15 μl of NEBuilder HiFi DNA Assembly Master Mix (NEB E2621), and nuclease-free water to a final volume of 30 μl, incubating at 50 °C for 1 h. The assembled plasmid was column-purified (Monarch PCR & DNA Cleanup Kit, NEB T1030) and then 25 ng of the column-purified plasmid was electroporated into 25 μl of Endura Electrocompetent Cells (Lucigen 60242-1) using a Gene Pulser II (Bio-Rad) under the following conditions: 10 μF capacitance, 600 Ohms resistance, 1.8

    Polymerase Chain Reaction:

    Article Title: Clonal dynamics shaped by diverse drug-tolerant persister states in melanoma resistance.
    Article Snippet: .. We digested the vector for 5 h with KpnI (NEB), gel-purified the linearized vector, and performed Gibson Assembly using 30 fmol of vector, 160 fmol of barcode oligonucleotides (100 nM in nuclease-free water), 15 μl of NEBuilder HiFi DNA Assembly Master Mix (NEB E2621), and nuclease-free water to a final volume of 30 μl, incubating at 50 °C for 1 h. The assembled plasmid was column-purified (Monarch PCR & DNA Cleanup Kit, NEB T1030) and then 25 ng of the column-purified plasmid was electroporated into 25 μl of Endura Electrocompetent Cells (Lucigen 60242-1) using a Gene Pulser II (Bio-Rad) under the following conditions: 10 μF capacitance, 600 Ohms resistance, 1.8 .. We digested the vector for 5 h with KpnI (NEB), gel-purified the linearized vector, and performed Gibson Assembly using 30 fmol of vector, 160 fmol of barcode oligonucleotides (100 nM in nuclease-free water), 15 μl of NEBuilder HiFi DNA Assembly Master Mix (NEB E2621), and nuclease-free water to a final volume of 30 μl, incubating at 50 °C for 1 h. The assembled plasmid was column-purified (Monarch PCR & DNA Cleanup Kit, NEB T1030) and then 25 ng of the column-purified plasmid was electroporated into 25 μl of Endura Electrocompetent Cells (Lucigen 60242-1) using a Gene Pulser II (Bio-Rad) under the following conditions: 10 μF capacitance, 600 Ohms resistance, 1.8



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